Phagocytic Cells & Their Function

Table of Contents

Lecture 1

Phagocytic Cells & Their Function

Purdue University Cancer Center&Purdue University Cytometry Laboratories

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Flow cytometry measurements

Presentation Overview

Role of Neutrophils

Factors that Increase PMN #s

Mechanisms of Action

General Pathway

We can monitor phagocytic cells

Scatter Pattern of Human leukocytes

Opsonins

IgG Receptors

Fc_ RI (CD64)

Fc_ RII (CD32)

Fc_ RIII (CD16)

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Fc_ R mediated Phagocytosis

Summary of Fc

Phagocytosis

Trypan Blue is added to remove the external fluorescence

Complement Related Proteins

Neutrophil Complement Receptors

Complement R CR3

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CR3

Destruction & Killing

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Membrane Complexes

NADPH Oxidase of Neutrophils

The Oxidase

Major Differences in Phagocytic Cells

Other Oxidative systems

Oxidative Reactions

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Summary

Acknowledgements

Thank you for your attention

Email: jpr@flowcyt.cyto.purdue.edu

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